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Goat Anti-TRIM35 / MAIR Antibody (EB05926)

Code Name Applications Availability Size Tested species Price Grade  
EB05926 Goat Anti-TRIM35 / MAIR Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB05926 1
EB05926 (1µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: TRIM35, MAIR, KIAA1098, MGC17233, tripartite motif-containing 35
Official Symbol: TRIM35
Accession Number(s): NP_741983.2
Human GeneID(s): 23087

Immunogen

Peptide with sequence ERSPDVSPGPSRS-C, from the N Terminus of the protein sequence according to NP_741983.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 50kDa band observed in lysates of cell line K562 (calculated MW of 56.5kDa according to NP_741983.2). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human