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Goat Anti-TRPC4 Antibody (EB08796)

Related Products: EBP08796 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08796 Goat Anti-TRPC4 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08796 1
EB08796 (0.3µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08796 2
EB08796 (4.75µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08796 3
EB08796 (4.75µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: TRPC4, transient receptor potential cation channel, subfamily C, member 4, HTRP4, MGC119570, MGC119571, MGC119572, MGC119573, TRP4, transient receptor potential 4
Official Symbol: TRPC4
Accession Number(s): NP_057263.1; NP_003297.1; NP_001129427.1; NP_001129429.1; NP_001129428.1; NP_001129430.1
Human GeneID(s): 7223
Non-Human GeneID(s): 22066 (mouse), 84494 (rat)
Important Comments: This antibody is expected to recognise all six reported isoforms (NP_057263.1; NP_003297.1; NP_001129427.1; NP_001129429.1; NP_001129428.1; NP_001129430.1).

Immunogen

Peptide with sequence C-KHAKEEDSSIDYD, from the internal region (near C Terminus) of the protein sequence according to NP_057263.1; NP_003297.1; NP_001129427.1; NP_001129429.1; NP_001129428.1; NP_001129430.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 110kDa band observed in Rat Brain lysates (calculated MW of 112kDa according to Human NP_057263.1 and to Rat NP_536321.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. This antibody has been successfully used in Western blot on Mouse, PMID: 26316271 and PMID: 25016017. This antibody has been successfully used in Western blot on Human, PMID: 25016017.
IHC: Paraffin embedded Human Placenta and Kidney. Recommended concentration: 4.75µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Specific References

This antibody has been successfully used in Western blot on Mouse:
Tauseef M, Farazuddin M, Sukriti S, Rajput C, Meyer JO, Ramasamy SK, Mehta D.
Transient receptor potential channel 1 maintains adherens junction plasticity by suppressing sphingosine kinase 1 expression to induce endothelial hyperpermeability.
FASEB J. 2016 Jan;30(1):102-10
PMID: 26316271

This antibody has been successfully used in Western blot on Human and Mouse:
Auditi DebRoy, Stephen M. Vogel, Dheeraj Soni, Premanand C. Sundivakkam, Asrar B. Malik, and Chinnaswamy Tiruppathi.
Cooperative signaling via transcription factors NF-κB and AP1/c-Fos mediates endothelial cell STIM1 expression and hyperpermeability in response to endotoxin.
J Biol Chem. 2014 Aug 29;289(35):24188-201
PMID: 25016017

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