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Goat Anti-TUBB3 Antibody (EB11685)

Related Products: EBP11685 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11685 Goat Anti-TUBB3 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB11685 1
EB11685 (2µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: beta-4, CDCBM, CFEOM3A, class III beta-tubulin, OTTHUMP00000176846, TUBB3, TUBB4, tubulin beta-3 chain, tubulin beta-4 chain, tubulin beta-III, tubulin, beta 3
Official Symbol: TUBB3
Accession Number(s): NP_006077.2
Human GeneID(s): 10381
Non-Human GeneID(s): 22152 (mouse), 246118 (rat)
Important Comments: This antibody is expected to recognize isoform 1 (NP_006077.2) only.

Immunogen

Peptide with sequence C-DPSGNYVGDSD, from the internal region (near N terminus) of the protein sequence according to NP_006077.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 52kDa band observed in lysates of cell line HepG2 (calculated MW of 50.4kDa according to NP_006077.2). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

Specific Reference

This antibody has been successfully used in the following paper:
Bismark Appiah et al.
DOT1L activity affects neural stem cell division mode and reduces differentiation and ASNS expression.
EMBO Rep. 2023 Aug; 24(8): e56233.
PMID: 37382163

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