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Code Name Applications Availability Size Tested species Price Grade  
EB08231 Goat Anti-UXT Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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EB08231 1
EB08231 (0.03µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB08231 2
EB08231 (5µg/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: UXT, ubiquitously-expressed transcript protein, ART-27, androgen receptor trapped clone 27 protein, SKP2-associated alpha PFD 1, STAP1, ubiquitously expressed prefoldin like chaperone, ubiquitously-expressed, prefoldin-like chaperone
Official Symbol: UXT
Accession Number(s): NP_004173.1; NP_705582.1
Human GeneID(s): 8409
Important Comments: This antibody is expected to recognize both reported isoforms (NP_004173.1; NP_705582.1).

Immunogen

Peptide with sequence C-DHRDKVYEQLAKY, from the C Terminus of the protein sequence according to NP_004173.1; NP_705582.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 20kDa band observed in Human Kidney and Skeletal Muscle lysates (calculated MW of 19.6kDa according to NP_705582.1). Recommended concentration: 0.01-0.03µg/ml.
IHC: In paraffin embedded Thyroid Gland shows staining of cystoplasm in all epithelial cells, but nuclear staining in some of them. Recommended concentration, 5-10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Specific Reference

This antibody has been successfully used in Western blot:
Mingyu Pan, Yue Yin, Tongyu Hu, Xinxia Wang, Tian Jia, Jing Sun, Quanyi Wang, Wei Meng, Juanjuan Zhu, Chunsun Dai, Haiyang Hu, Chen Wang
UXT attenuates the CGAS-STING1 signaling by targeting STING1 for autophagic degradation.
Autophagy. 2023 Feb;19(2):440-456.
PMID: 35543189

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