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Code Name Applications Availability Size Tested species Price Grade  
EB06324 Goat Anti-VDAC2 (C Terminus) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Rat $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06324 1
EB06324 (0.3µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06324 2
EB06324 (1ug/ml) staining of CaCo-2 (A) and (0.5ug/ml) LNCaP (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06324 3
EB06324 (6µg/ml) staining of paraffin embedded Rat Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB06324 4
EB06324 Negative Control showing staining of paraffin embedded Rat Liver, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: VDAC2, voltage-dependent anion channel 2, RP11-375G3.1, FLJ23841
Official Symbol: VDAC2
Accession Number(s): NP_003366.2
Human GeneID(s): 7417

Immunogen

Peptide with sequence C-GHKVGLALELEA, from the C Terminus of the protein sequence according to NP_003366.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 35kDa band observed in Human Cerebellum and in lysates of cell lines CaCo-2 and LNCaP (calculated MW of 31.6kDa according to Human NP_003366.2). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Rat Liver. Recommended concentration: 5-6µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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