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Code Name Applications Availability Size Tested species Price Grade  
EB10811 Goat Anti-ZIC3 (aa178-189) Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10811 1
EB10811 (5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ZIC3, Zic family member 3 (odd-paired homolog, Drosophila), HTX, HTX1, ZNF203, heterotaxy 1, zinc finger protein ZIC 3, zinc finger protein of the cerebellum 3
Official Symbol: ZIC3
Accession Number(s): NP_003404.1
Human GeneID(s): 7547
Non-Human GeneID(s): 22773 (mouse), 367944 (rat)
Important Comments: This antibody is expected to NOT cross-react with other ZIC proteins.

Immunogen

Peptide with sequence C-HVDNNQVHLGLR, from the internal region of the protein sequence according to NP_003404.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary experiments gave an approx 70kDa band in Human, Mouse and Rat Brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 50.6kDa according to NP_003404.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC: In paraffin embedded Human Prostate shows nuclear staining in select secretory cells. Recommended concentration, 5-10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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