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Goat Anti-ZNRF1 (mouse) Antibody (EB11598)

Related Products: EBP11598 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11598 Goat Anti-ZNRF1 (mouse) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB11598 1
N2a overexpressing Mouse Znrf1 (mock transfection in first lane) and probed with EB11598 (1µg/ml), also staining of Mouse Brain lysates (Embryo E14 and adult cerebellum). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11598 2
EB11598 (10µg/ml) staining of paraffin embedded Mouse Cerebral Cortex. Microwaved antigen retrieval with citrate buffer pH 6, streptavidfine-Alexa 488-staining after biotinylated anti-goat secondary. The Neurofilament M was labeled by Millipore AB1987 (1:100).
EB11598 3
EB11598 (5µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Target Protein

Principal Names: B830022L21Rik, E3 ubiquitin-protein ligase ZNRF1, MGC101991, nerve injury-induced gene 283 protein, nin283, peripheral nerve injury protein nin283, ring finger protein 42, Rnf42, zinc and ring finger 1, zinc ring finger protein 1, zinc/RING finger protein 1, Znrf1, Zrfp1
Official Symbol: Znrf1
Accession Number(s): NP_573469.1; NP_001162093.1
Human GeneID(s): 84937
Non-Human GeneID(s): 170737 (mouse), 690769 (rat)
Important Comments: This antibody is expected to recognize reported isoforms a and b (NP_573469.1; NP_001162093.1). Reported variants represent identical protein: NP_001162092.1, NP_573469.1

Immunogen

Peptide with sequence C-SDSTYAHGNGYQET, from the internal region of the protein sequence according to NP_573469.1; NP_001162093.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 28kDa band observed in Mouse embryo and adult Brain lysates (right panel), consistent with the observed band in transfected N2a cells transiently expressing Znrf1(left panel) Calculated MW of 23.8kDa according to NP_573469.1. Recommended concentration: 1-3µg/ml. Data obtained from Dr. Shuji Wakatsuki, National Institute of Neuroscience, Tokyo, Japan
IHC: In paraffin embedded Mouse Cerebral Cortex shows colabeling with Neurofilament M. Recommended concentration, 10µg/ml. Data obtained from Dr. Shuji Wakatsuki, National Institute of Neuroscience, Tokyo, Japan. Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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