Goat Anti-ACAT1 (aa253-266) Antibody

$464.00

Category: Primary Antibodies
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SKU: EB09741
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
mitochondrial acetoacetyl-CoA thiolase|acetyl-CoA acetyltransferase 1|acetoacetyl Coenzyme A thiolase|THIL|T2|MAT|ACAT|acetyl-Coenzyme A acetyltransferase 1|ACAT1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and A431 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_000010.1
Blocking Peptide
EBP09741
Immunogen
Peptide with sequence C-DEEYKRVDFSKVPK, from the internal region of the protein sequence according to NP_000010.1.
Peptide Sequence
C-DEEYKRVDFSKVPK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human, Mouse, Rat
Human Gene ID
38
Mouse Gene ID
110446
Rat Gene ID
25014
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:4000.
Western Blot
Approx. 40kDa band observed in lysates of cell lines CAC02, HEK293, HepG2, MCF7, NIH3T3 and KNRK and approx. 38-40kDa in Human, Mouse and Rat Liver lysates (calculated MW of 45.2kDa according to Human NP_000010.1, 44.81kDa according to Mouse NP_659033.1 and 44.7kDa according to Rat NP_058771.2 ). Recommended concentration: 0.01-0.5µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC

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