Goat Anti-Actin-like 7B (mouse) Antibody

$423.00

Category: Primary Antibodies
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SKU: EB07651
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
t-actin 1|actin-like 7-beta|MGC151192, MGC151194, Tact1|ACTL7B|actin-like 7B
Accession ID
NP_079547.1
Blocking Peptide
EBP07651
Immunogen
Peptide with sequence C-DELHVDYELPDGK, from the internal region of the protein sequence according to NP_079547.1.
Product Comments
This antibody is not expected to cross-react with Actin-like 7A.
Peptide Sequence
C-DELHVDYELPDGK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Mouse, Rat
Reactive Species
Rat
Mouse Gene ID
11471
Rat Gene ID
313183
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 45kDa band observed in Rat Testis lysates (calculated MW of 45.5kDa according to Rat NP_001020588.1 and 45.6kDa according to Mouse NP_079547.1). Recommended concentration: 0.5-1.5µg/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Application Type
Pep-ELISA, WB

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