Goat Anti-ACTN1 (aa596-609) Antibody

$423.00

Category: Primary Antibodies
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SKU: EB12420
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
non-muscle alpha-actinin-1|alpha-actinin-1|alpha-actinin cytoskeletal isoform|actinin 1 smooth muscle|F-actin cross-linking protein|actinin, alpha 1|ACTN1
Accession ID
NP_001123476.1; NP_001093.1; NP_001123477.1
Blocking Peptide
EBP12420
Immunogen
Peptide with sequence C-TPQEINGKWDHVRQ, from the internal region of the protein sequence according to NP_001123476.1; NP_001093.1; NP_001123477.1.
Product Comments
This antibody is expected to recognize all reported isoforms (NP_001123476.1; NP_001093.1; NP_001123477.1).
Peptide Sequence
C-TPQEINGKWDHVRQ
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Pig, Cow
Reactive Species
Human, Mouse, Rat
Human Gene ID
87
Mouse Gene ID
109711
Rat Gene ID
81634
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 100kDa band observed in Human, Mouse and Rat Spleen lysates (calculated MW of 103kDa according to NP_001093.1). Recommended concentration: 0.3-1µg/ml. An additional band of unknown identity was also consistently observed at 75kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Application Type
Pep-ELISA, WB

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