Goat Anti-AF10 / MLLT10 Antibody

$255.00

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SKU: EB05825
Unit Size
100 µg
Synonym / Alias Names
type I AF10 protei|OTTHUMP00000045273|MGC75086|DKFZp686E10210|RP11-399C16.2|myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog) translocated to, 10|ALL1 fused gene from chromosome 10|AF10|myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila) translocated to, 10|MLLT10
Accession ID
NP_004632.1; NP_001009569.1
Immunogen
Peptide with sequence C-HFSPSMEIMQVRK, from the C Terminus of the protein sequence according to NP_004632.1; NP_001009569.1.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_004632.1; NP_001009569.1.
Peptide Sequence
C-HFSPSMEIMQVRK
Shipping Instructions
Refrigerated
Predicted Species
Human
Human Gene ID
8028
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Western Blot: experiments gave bands at approx 85kDa, 60kDa and 26kDa in Human Placenta lysate after 1µg/ml antibody staining. These detected bands were successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in the literature for the bands we observe given the predicted sizes of 109kDa according to NP_004632 for isoform a and 107kDa according to NP_001009569 for isoform b. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Application Type
Pep-ELISA