Goat Anti-AIF1/IBA1 isoform 1 and 3 Antibody

$464.00

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SKU: EB05419
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
protein G1|IRT1|allograft inflammatory factor-1 splice variant Hara-1|DADB-70P7.8|Daintain|ionized calcium-binding adapter molecule 1|interferon gamma responsive transcript|IRT-1|AIF-1|allograft inflammatory factor 1|IBA1|AIF1
Accession ID
NP_116573.1; NP_001614.3
Blocking Peptide
EBP05419
Immunogen
Peptide with sequence C-TGPPAKKAISELP, from the C Terminus of the protein sequence according to NP_116573.1; NP_001614.3.
Product Comments
This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).
Peptide Sequence
C-TGPPAKKAISELP
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Pig
Reactive Species
Human, Mouse, Rat
Human Gene ID
199
Mouse Gene ID
11629
Rat Gene ID
29427
Product Grade
IHC Results
Paraffin embedded Human Spleen and Lung. Recommended concentration: 8µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 16kDa band observed in Mouse and Rat Brain lysates (calculated MW of 16.9kDa according to Mouse NP_062340.1 and 16.8kDa according to Rat NP_058892.1) . Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
Application Type
Pep-ELISA, WB, IHC
Selected References
[{"pmid": 21294954, "intro": "This antibody (previous batch) has been successfully used in IHC on Mouse:", "title": "Bone marrow contributes simultaneously to different neural types in the central nervous system through different mechanisms of plasticity.", "author": "Recio JS, Álvarez-Dolado M, Díaz D, Baltanás FC, Piquer-Gil M, Alonso JR, Weruaga E.", "journal": "Cell Transplant. 2011;20(8):1179-92."}]