Goat Anti-AIF1 / IBA1 (isoforms 1 + 3) Antibody

$464.00

Category: Primary Antibodies
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SKU: EB07257
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
ionized calcium-binding adapter molecule|interferon gamma responsive transcript|Em:AF129756.17|IRT-1|AIF-1|DASS-82G15.2|allograft inflammatory factor 1|IBA1|AIF1
Usage Summary
Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of CaCo-2 cells. Recommended concentration: 10µg/ml.
Accession ID
NP_116573.1; NP_001614.3
Blocking Peptide
EBP07257
Immunogen
Peptide with sequence C-YEEKAREKEKP, from the C Terminus of the protein sequence according to NP_116573.1; NP_001614.3.
Product Comments
This antibody is expected to recognise isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).
Peptide Sequence
C-YEEKAREKEKP
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
199
Product Grade
IHC Results
In paraffin embedded Human Cerebellum shows staining of astrocytes. Recommended concentration, 2-4µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Application Type
Pep-ELISA, FC, IF, IHC

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