Goat Anti-AKAP9 / AKAP450 / CG-NAP Antibody

$423.00

Category: Primary Antibodies
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SKU: EB06654
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
AKAP9-BRAF fusion protein|A-kinase anchor protein 9|MU-RMS-40.16A|AKAP450|centrosome- and golgi-localized protein|protein kinase A anchoring protein 9|A-kinase anchor protein, 350kDa|A-kinase anchoring protein 450|kinase N-associated protein|AKAP120-like protein|A kinase (PRKA) anchor protein (yotiao) 9|KIAA0803|HYPERION|AKAP350|YOTIAO|PRKA9|CG-NAP|AKAP9
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10µg/ml.
Accession ID
NP_005742.4; NP_671714.1
Blocking Peptide
EBP06654
Immunogen
Peptide with sequence C-SGSTTQFHAGMR, from the C Terminus of the protein sequence according to NP_005742.4; NP_671714.1.
Product Comments
This antibody is expected to recognise isoforms 2 and 3 (NP_005742.4; NP_671714.1 respectively). The isoforms Yotiao, AKAP350B and AKAP350C are not recognized.
Peptide Sequence
C-SGSTTQFHAGMR
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse
Reactive Species
Human
Human Gene ID
10142
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
This product has been successfully used by a customer, showing a band at approx 450kDa in lysates of cell line HepG2 (calculated MW of 453kDa according to NP_005742.4). Recommended concentration: 1-3µg/ml.
Application Type
Pep-ELISA, WB, IF

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