Goat Anti-AKT1 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB07691
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
rac protein kinase alpha|protein kinase B|murine thymoma viral (v-akt) oncogene homolog-1|RAC-alpha serine/threonine-protein kinase|RAC-ALPHA|RAC|PRKBA|PKB|MGC99656|AKT|v-akt murine thymoma viral oncogene homolog 1|AKT1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the microtubules/cytoplasm of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_005154.2; NP_001014431.1; NP_001014432.1
Blocking Peptide
EBP07691
Immunogen
Peptide with sequence C-QDVDQREAPLN, from the internal region of the protein sequence according to NP_005154.2; NP_001014431.1; NP_001014432.1.
Product Comments
All variants represent identical protein (NP_005154.2, NP_001014431.1, NP_001014432.1). This antibody is NOT expected to cross-react with AKT2. It may recognize AKT1 in Rat and Dog.
Peptide Sequence
C-QDVDQREAPLN
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse
Reactive Species
Human
Human Gene ID
207
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 60kDa band observed in MCF7 cell lysates and approx. 55kDa in preliminary testing of K562 and NIH3T3 cell lysate (calculated MW of 55.7kDa according to NP_005154.2). These molecular weights are observed by other commercial sources. Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC

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