Goat Anti-AKT1 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB06875
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
rac protein kinase alpha|protein kinase B|murine thymoma viral (v-akt) oncogene homolog-1|RAC-alpha serine/threonine-protein kinase|RAC-ALPHA|RAC|PRKBA|PKB|MGC99656|HGNC:391|v-akt murine thymoma viral oncogene homolog 1|AKT1
Accession ID
NP_005154.2; NP_001014432.1; NP_001014431.1
Blocking Peptide
EBP06875
Immunogen
Peptide with sequence C-EVSLAKPKHR, from the internal region of the protein sequence according to NP_005154.2; NP_001014432.1; NP_001014431.1.
Product Comments
Variants one, two and three encode the same protein (NP_005154, NP_001014432, NP_001014431). No cross-reactivity expected with AKT2 or AKT3.
Peptide Sequence
C-EVSLAKPKHR
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Cow
Reactive Species
Human, Mouse
Human Gene ID
207
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx 60kDa band observed in MCF7 and approx. 60-65kDa in NIH3T3 cell lysates (calculated MW of 55.7kDa according to Human NP_005154.2 and Mouse NP_033782.1). This molecular weight is routinely observed by other sources. Recommended concentration: 2-3ug/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB

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