Goat Anti-AKT2 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB06772
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
rac protein kinase beta|Murine thymoma viral (v-akt) homolog-2|RAC-BETA|PRKBB|PKBBETA|HGNC:392|v-akt murine thymoma viral oncogene homolog 2|AKT2
Usage Summary
Flow Cytometry: A customer reported weak staining on THP-1 cells at a concentration of 10ug/ml.
Accession ID
NP_001617.1; NP_001229956.1; NP_001317440.1
Blocking Peptide
EBP06772
Immunogen
Peptide with sequence C-RYDSLGLLELDQRT, from the C Terminus of the protein sequence according to NP_001617.1; NP_001229956.1; NP_001317440.1.
Product Comments
This antibody is expectesd to recognise all reported iasoforms NP_001617.1; NP_001229956.1; NP_001317440.1.
Peptide Sequence
C-RYDSLGLLELDQRT
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog
Reactive Species
Human, Rat
Human Gene ID
208
Product Grade
IHC Results
Paraffin embedded Human Testis. Recommended concentration: 4-6µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx. 58-60kDa band observed in Human Skeletal Muscle lysates and in Rat Testis lysates, and approx.60kDa in Human Testis lysates, and in preliminary testing of THP-1 cell lysate (calculated MW of 55.8kDa according to Human NP_001617.1 and 55.5kDa according to Rat NP_058789.1). Recommended concentration: 0.5-1µg/ml. Primary incubation 1 hour at room temperature
Application Type
Pep-ELISA, WB, IHC

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