Goat Anti-AKT3 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

RAC-gamma serine/threonine protein kinase|serine threonine protein kinase, Akt-3|protein kinase B gamma|v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)|DKFZP434N0250|RAC-PK-gamma|RAC-gamma|STK-2|PRKBG|PKBG|AKT3

Immunofluorescence: Strong expression of the protein seen in THP-1 and A431 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

NP_005456.1; NP_859029.1

EBP06562

Peptide with sequence CSPTSQIDNIGEEEM, from the internal region of the protein sequence according to NP_005456.1; NP_859029.1.

This antibody is expected to recognise an epitope corresponding to aa 119-133 of both isoforms of human AKT3 protein. This antibody does not cross-react with human AKT1/2. Reported variants represent identical protein (NP_859029.1; NP_001193658.1).

CSPTSQIDNIGEEEM

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog, Cow

Human, Mouse, Rat

10000

Paraffin embedded Human Prostate and Heart. Recommended concentration: 5µg/ml.

Antibody detection limit dilution 1:16000.

Approx. 50-55kDa band observed in lysates of cell lines HepG2, and HeLa , approx. 55kDa in Human Thyroid lysates, and approx. 50kDa in Mouse and Rat Brain lysates (calculated MW of 54.0kDa according to Human NP_859029.1 and 55.7kDa according to Mouse NP_035915.3 and Rat NP_113763.2). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.

Positive Control: A batch specific positive control lysate is available for this product. Please contact [email protected] for availability.

Pep-ELISA, WB, IHC, IF, FC