Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names retinaldehyde dehydrogenase 1|retinal dehydrogenase 1|aldehyde dehydrogenase, liver cytosolic|aldehyde dehydrogenase 1A1|aldehyde dehydrogenase 1, soluble|acetaldehyde dehydrogenase 1|ALDH class 1|RALDH1|PUMB1|MGC2318, |ALDH11|ALDH1|ALDH-E1|ALDC|aldehyde dehydrogenase 1 family, member A1|ALDH1A1 | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 10µg/ml. This antibody has been successfully used in IF on Human, PMID: 32157826. | |
Accession ID NP_000680.2 | |
Blocking Peptide EBP05049 | |
Immunogen Peptide with sequence C-EVKTVTVKISQKNS, from the C Terminus of the protein sequence according to NP_000680.2. | |
Product Comments This antibody may cross-react with ALDH1A2 (GeneID 8854) with one residue difference from this design and with ALDH2 (GeneID 217) with two residues difference from this design. | |
Peptide Sequence C-EVKTVTVKISQKNS | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat | |
Reactive Species Human, Mouse, Rat | |
Human Gene ID 216 | |
Mouse Gene ID 11668 | |
Rat Gene ID 24188 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:1000. | |
Western Blot Approx 55kDa band observed in Human, Mouse and Rat Liver lysates (calculated MW of 54.9kDa according to NP_000680.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour. | |
Application Type Pep-ELISA, WB, IF |
Goat Anti-ALDH1A1 (C Terminus) Antibody
$423.00
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Selected References [{"pmid": 22020955, "intro": "This antibody (previous batch) has been successfully used in WB and ICC on Human:", "title": "Expression and activity of alcohol and aldehyde dehydrogenases in melanoma cells and in melanocytes.", "author": "Amann PM, Hofmann C, Freudenberger M, Holland-Cunz S, Eichmüller SB, Bazhin AV.", "journal": "J Cell Biochem. 2012 Mar;113(3):792-9."}, {"pmid": 32157826, "intro": "This antibody has been successfully used in IF on Human:", "title": "Aldehyde dehydrogenases contribute to skeletal muscle homeostasis in healthy, aging, and Duchenne muscular dystrophy patients.", "author": "Jessy Etienne, Pierre Joanne, Cyril Catelain, Stéphanie Riveron, Alexandra Clarissa Bayer, Jérémy Lafable, Isabel Punzon, Stéphane Blot, Onnik Agbulut, and Jean?Thomas Vilquin", "journal": "J Cachexia Sarcopenia Muscle. 2020 Aug; 11(4): 1047-1069."}] |