Goat Anti-Alpha-synuclein (NACP140) Antibody

$423.00

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SKU: EB11714
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
synuclein, alpha (non A4 component of amyloid precursor)|synuclein alpha-140|SNCA|PD1|PARK4|PARK1|non A-beta component of AD amyloid|NACP|alpha-synuclein
Accession ID
NP_000336.1
Blocking Peptide
EBP11714
Immunogen
Peptide with sequence C-KDQLGKNEEGAPQE, from the internal region of the protein sequence according to NP_000336.1.
Product Comments
This antibody is expected to recognize reported isoform NACP140 (NP_000336.1) only. Reported variants represent identical protein: NP_000336.1, NP_001139527.1, NP_001139526.1
Peptide Sequence
C-KDQLGKNEEGAPQE
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Pig
Reactive Species
Human
Human Gene ID
6622
Product Grade
IHC Results
Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 17kDa band observed in Human Brain (Amygdala) lysates (calculated MW of 14.5kDa according to NP_000336.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
Application Type
Pep-ELISA, WB, IHC
Selected References
[{"pmid": 25634539, "intro": "This antibody has been successfully used in WB and IF on Rat:", "title": "DT-Diaphorase Prevents Aminochrome-Induced Alpha-Synuclein Oligomer Formation and Neurotoxicity.", "author": "Muñoz P, Cardenas S, Huenchuguala S, Briceño A, Couve E, Paris I, Segura-Aguilar J.", "journal": "Toxicol Sci. 2015 May;145(1):37-47."}]