Goat Anti-ALS2CR2 / ILPIP Antibody

$464.00

Category: Primary Antibodies
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SKU: EB05865
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
pseudokinase ALS2CR2|STRAD beta|STE20-related kinase adaptor beta|ILP-interacting protein ILPIPA|amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 2|PRO1038|CALS-21|ILPIPA|PAPK|ILPIP|ALS2CR2|STRADB
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U251 cells. Recommended concentration: 10µg/ml.
Accession ID
NP_061041.2; AAF71042.1
Blocking Peptide
EBP05865
Immunogen
Peptide with sequence CDFPDEKDSYWEF, from the C Terminus of the protein sequence according to NP_061041.2; AAF71042.1.
Product Comments
This is expected to recognise both human isoforms: ILPIP-alpha (NP_061041.2) and ILPIP-beta (AAF71042.1).
Peptide Sequence
CDFPDEKDSYWEF
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Dog, Cow
Reactive Species
Human
Human Gene ID
55437
Product Grade
IHC Results
In paraffin embedded Human Heart shows staining of myocardial fibres in transverse section. Recommended concentration: 3-5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 35kDa band observed in Human Heart, Liver and Muscle lysates (calculated MW of 47.0kDa according to NP_061041.2 (alpha isoform) and 31.2kDa according to AAF71042.1 (beta isoform). In transfected HEK293 transiently expressing ILPIP a band of approx. 48kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3µg/ml.
Application Type
Pep-ELISA, WB, IHC, IF

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