Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names scra|DKFZp779A055|anillin, actin binding protein|anillin (Drosophila Scraps homolog), actin binding protein|anillin, actin binding protein (scraps homolog, Drosophila)|ANILLIN|Scraps|ANLN | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml. Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. | |
Accession ID NP_061155.2; NP_001271230.1; NP_001271231.1 | |
Blocking Peptide EBP06104 | |
Immunogen Peptide with sequence WQPDACYKPIGKP, from the C Terminus of the protein sequence according to NP_061155.2; NP_001271230.1; NP_001271231.1. | |
Peptide Sequence WQPDACYKPIGKP | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Cow | |
Reactive Species Human | |
Human Gene ID 54443 | |
Mouse Gene ID 68743 | |
Product Grade  | |
IHC Results In paraffin embedded Human Kidney shows staining of nuclei in some cells of renal tubules. Recommended concentration: 3-10µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:64000. | |
Application Type Pep-ELISA, IHC, IF, FC |
Goat Anti-ANILLIN / Scraps (C Terminus) Antibody
$464.00
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Selected References [{"pmid": 30377371, "intro": "This antibody has been successfully used in the following paper:", "title": "A high-throughput pipeline for validation of antibodies", "author": "Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen", "journal": "Nat Methods. 2018 Nov;15(11):909-912"}] |