Goat Anti-APOM Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

APOM|apolipoprotein M|G3a|HSPC336|MGC22400|NG20|NG20-like protein|G3a|NG20

NP_061974.2

EBP07578

Peptide with sequence PRNQEACELSNN, from the C Terminus of the protein sequence according to NP_061974.2.

PRNQEACELSNN

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Dog

Human

55937

In paraffin embedded Human Liver shows strong stain of cytoplasm in select hepatocytes. Recommended concentration, 3-6µg/ml.

Antibody detection limit dilution 1:8000.

Preliminary experiments gave an approx 50kDa band in Human Kidney and Liver lysates after 0.2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 21.3kDa according to NP_061974.2. We do note that ApoM is a glycoprotein which makes a higher size band to be expected. The 50kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA, IHC