Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names ARIH2|ariadne homolog 2 (Drosophila)|ARI2|TRIAD1|all-trans retinoic acid inducible RING finger|ariadne-2 (D. melanogaster) homolog (all-trans retinoic acid inducible RING finger)|FLJ10938|FLJ33921|OTTHUMP00000210388|ariadne homolog 2 | |
Usage Summary Immunofluorescence: Customer's experiments showed nuclear staining in DAPI with IF. This antibody has also been successfully used in IF in PMID: 24486325. | |
Accession ID NP_006312.1 | |
Blocking Peptide EBP05813 | |
Immunogen Peptide with sequence C-EQRRRTLLKDFHDT, from the C Terminus of the protein sequence according to NP_006312.1. | |
Peptide Sequence C-EQRRRTLLKDFHDT | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Cow | |
Reactive Species Human, Mouse | |
Human Gene ID 10425 | |
Mouse Gene ID 23807 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:8000. | |
Western Blot Approx 60kDa band observed in nuclear lysates of Hela and K562 (Calculated MW of 57.8kDa according to NP_006312.1) Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.
Customer's experiments gave a band at approx 60kDa in lysates in both Human HeLa and Mouse Muscle (IM2 & C2C12) cells. This band disappeared after RNAi knock-down. Calculated MW of 57.8kDa according to NP_006312.1). Recommended concentration: 1-3µg/ml. This antibody has also been successfully used in WB in PMID: 24486325. | |
Application Type Pep-ELISA, WB, IF |
Goat Anti-ARIH2 / TRIAD1 Antibody
$423.00
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Selected References [{"pmid": 24486325, "intro": "This antibody has been successfully used in WB and IF on Human:", "title": "A novel feed-forward loop between ARIH2 E3 ligase and PABPN1 regulates aging-associated muscle degeneration.", "author": "Raz V, Buijze H, Raz Y, Verwey N, Anvar SY, Aartsma-Rus A, van der Maarel SM.", "journal": "Am J Pathol. 2014 Apr;184(4):1119-31."}] |