Goat Anti-Arylsulfatase B Antibody

$423.00

Category: Primary Antibodies
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SKU: EB08059
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
N-acetylgalactosamine-4-sulfatase|MPS6|G4S|ASB|arylsulfatase B|ARSB
Accession ID
NP_000037.2; NP_942002.1
Blocking Peptide
EBP08059
Immunogen
Peptide with sequence C-KLARGHTNGTKPLD, from the internal region of the protein sequence according to NP_000037.2; NP_942002.1.
Product Comments
This antibody is expected to recognise both reported isoforms (NP_000037.2; NP_942002.1).
Peptide Sequence
C-KLARGHTNGTKPLD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
411
Product Grade
IHC Results
In paraffin embedded Human Skeletal Muscle shows striated pattern staining of majority of muscular fibres. Recommended concentration, 5-10µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Approx 45kDa band observed in Human Heart lysates (calculated MW of 46.0kDa according to NP_942002.1). Recommended concentration: 0.03-0.1µg/ml. An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Application Type
Pep-ELISA, WB, IHC

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