Goat Anti-ATXN1 (aa686-699) Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

spinocerebellar ataxia type 1 protein| ataxin-1| OTTHUMP00000016065| SCA1| D6S504E| ATX1| ataxin 1|ATXN1

NP_000323.2

EBP10911

Peptide with sequence C-TLKNLKNGSVKKGQ, from the internal region of the protein sequence according to NP_000323.2.

Reported variants represent identical protein: NP_001121636.1, NP_000323.2

C-TLKNLKNGSVKKGQ

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat

6310

20238

25049

Antibody detection limit dilution 1:32000.

Preliminary experiments gave bands at approx 75kDa and 26kDa in Mouse Fetal Brain lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 84.7kDa according to NP_001186234.1 and 83.8kDa according to NP_033150.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA