Goat Anti-Atoh7 (mouse) Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

Atoh7|atonal homolog 7 (Drosophila)|Math|atonal homolog 7

NP_058560.1

EBP07972

Peptide with sequence CEQRGRDHPYLP, from the internal region of the protein sequence according to NP_058560.1.

CEQRGRDHPYLP

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Mouse, Rat

53404

365564

Antibody detection limit dilution 1:32000.

Preliminary experiments gave bands at approx 50kDa and 38kDa in Mouse Eye and Rat Optical Nerve lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 16.9kDa according to NP_058560.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA