Goat Anti-Atrial natriuretic factor Antibody

$423.00

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SKU: EB11166
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
NPPA| prepronatriodilatin| PND| natriuretic peptide precursor A| natriuretic peptide A| CDD-ANF| cardionatrin| atriopeptin| atrial natriuretic factor| ATFB6| ANP| ANF
Accession ID
NP_006163.1
Blocking Peptide
EBP11166
Immunogen
Peptide with sequence RIGAQSGLGCNSFR, from the C Terminus of the protein sequence according to NP_006163.1.
Product Comments
The immunizing peptide represents aa137-150 of the precursor NPPA.
Peptide Sequence
RIGAQSGLGCNSFR
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Pig, Cow
Reactive Species
Human, Mouse, Rat
Human Gene ID
4878
Mouse Gene ID
230899
Rat Gene ID
24602
Product Grade
IHC Results
In paraffin embedded Human Heart shows vesicular staining inside the cardiac fibrils, accumulating towards the cell membrane. Recommended concentration, 5-10µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 19kDa band observed in Mouse and Rat Heart lysates (calculated MW of 16.4kDa according to Human precursor NP_006163.1 and 16.6kDa accordiong to Mouse precursor NP_032751.1). Recommended concentration: 0.01-0.03µg/ml.
Application Type
Pep-ELISA, WB, IHC
Selected References
[{"pmid": 32112822, "intro": "This antibody has been successfully used in Western blot on Mouse:", "title": "Low-density lipoprotein receptor-related protein 1 deficiency in cardiomyocytes reduces susceptibility to insulin resistance and obesity", "author": "Benitez-Amaro A, Revuelta-López E, Bornachea O, Cedó L, Vea À, Herrero L, Roglans N, Soler-Botija C, de Gonzalo-Calvo D, Nasarre L, Camino-López S, García E, Mato E, Blanco-Vaca F, Bayes-Genis A, Sebastian D, Laguna JC, Serra D, Zorzano A, Escola-Gil JC, Llorente-Cortes V", "journal": "Metabolism. 2020 Feb 26;106:154191."}]