Goat Anti-Aurora kinase A / AURKA Antibody

$423.00

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SKU: EB05035
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
OTTHUMP00000166071|OTTHUMP00000031345|OTTHUMP00000031344|OTTHUMP00000031343|OTTHUMP00000031342|OTTHUMP00000031340|serine/threonine protein kinase 6|serine/threonine protein kinase 15|serine/threonine kinase 6|serine/threonine kinase 15|breast-tumor-amplified kinase|aurora/IPL1-like kinase|aurora-related kinase 1|aurora-A|IPL1-related kinase|STK7|STK6|STK15|MGC34538|BTAK|AURORA2|AURA|ARK1|AIK|aurora kinase A|AURKA
Usage Summary
Immunoprecipitation: This antibody was deemed fit for IP under native conditions (observations from a customer).

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Accession ID
NP_003591.2
Blocking Peptide
EBP05035
Immunogen
Peptide with sequence C-QNKESASKQS, from the C Terminus of the protein sequence according to NP_003591.2.
Product Comments
Reported variants represent identical protein: NP_003591.2; NP_940835.1; NP_940836.1; NP_940837.1; NP_940838.1; NP_940839.1
Peptide Sequence
C-QNKESASKQS
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
6790
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 45kDa band observed in lysates of cell line HeLa and Jurkat lysate (calculated MW of 45.8kDa according to NP_003591.2). Recommended concentration: 0.1-0.3µg/ml.
Application Type
Pep-ELISA, WB, IP
Selected References
[{"pmid": 30377371, "intro": "This antibody has been successfully used in the following paper:", "title": "A high-throughput pipeline for validation of antibodies", "author": "Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen", "journal": "Nat Methods. 2018 Nov;15(11):909-912"}]