Goat Anti-beta-2-microglobulin Antibody

$423.00

Category: Primary Antibodies
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SKU: EB11515
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
OTTHUMP00000161912|beta-2-microglobulin|beta-2-microglobin|beta chain of MHC class I molecules|B2M
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the plasma membranes and cytoplasm of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Accession ID
NP_004039.1
Blocking Peptide
EBP11515
Immunogen
Peptide with sequence QRTPKIQVYSRHP-C, from the N Terminus of the protein sequence according to NP_004039.1.
Peptide Sequence
QRTPKIQVYSRHP-C
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
567
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Preliminary testing showed a band at approx. 12-13kDa in Human Spleen lysate and in Jurkat, U251, U2OS, U937 and HeLa cell lysate after 0.3-1µg/ml antibody staining (calculated MW of 13.7kDa according to NP_004039.1). Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, IF, FC

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