Goat Anti-BIRC6 / Apollon (aa 4724 -4735) Antibody

$255.00

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SKU: EB10371
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
BIRC6|ubiquitin-conjugating BIR-domain enzyme apollon|KIAA1289|FLJ13786|FLJ13726|BRUCE|baculoviral IAP repeat-containing 6|APOLLON
Accession ID
NP_057336.3
Blocking Peptide
EBP10371
Immunogen
Peptide with sequence C-SREYDGNIRQAT, from the internal region of the protein sequence according to NP_057336.3.
Peptide Sequence
C-SREYDGNIRQAT
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Cow
Human Gene ID
57448
Mouse Gene ID
12211
Rat Gene ID
313876
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Preliminary experiments gave bands at approx 90kDa and 70kDa in lysates of cell lines A431 and HeLa after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 530kDa according to NP_057336.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Application Type
Pep-ELISA