Goat Anti-BLNK / SLP-65 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB05111
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
OTTHUMP00000020168|LY57|BLNK-S|Src homology 2 domain-containing leukocyte protein of 65 kDa|OTTHUMP00000020167|B-cell adapter containing a Src homology 2 domain protein|B-cell adapter containing a SH2 domain protein|B cell linker protein|SLP65|MGC111051|Ly57|BLNK-s|BASH|B-cell linker|SLP-65|BLNK
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the membranes of HepG2 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of Daudi cells. Recommended concentration: 10ug/ml.

Accession ID
NP_037446.1; NP_001107566.1; NP_001245369.1; NP_001245370.1; NP_001245371.1
Blocking Peptide
EBP05111
Immunogen
Peptide with sequence C-KDSTRLKYAVKVS, from the C Terminus of the protein sequence according to NP_037446.1; NP_001107566.1; NP_001245369.1; NP_001245370.1; NP_001245371.1.
Product Comments
This antibody is expected to recognize all reported isoforms.
Peptide Sequence
C-KDSTRLKYAVKVS
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Pig, Cow
Reactive Species
Human
Human Gene ID
29760
Mouse Gene ID
17060
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 70-75kDa band observed in lysates of cell line Daudi (calculated MW of 50.5kDa according to NP_037446.1). This molecular weight is routinely observed by other sources. Recommended concentration 0.3-1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC

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