Goat Anti-BOULE / BOLL Antibody

$423.00

Category: Primary Antibodies
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SKU: EB06074
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
boule|BOULE|bol, boule-like (Drosophila)|BOLL
Accession ID
NP_932074.1; NP_149019.1
Blocking Peptide
EBP06074
Immunogen
Peptide with sequence C-QPEPIKTVWSIHY, from the C Terminus of the protein sequence according to NP_932074.1; NP_149019.1.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_932074.1; NP_149019.1).
Peptide Sequence
C-QPEPIKTVWSIHY
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Dog, Cow
Reactive Species
Human
Human Gene ID
66037
Product Grade
IHC Results
In paraffin embedded Human Testis shows cytoplasm staining in spermatocytes, but not in spermatogonia. Recommended concentration, 4-6µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Preliminary experiments gave an approx 22kDa band in Human Testis lysates after 1.5 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.6kDa according to NP_932074.1 and 31.3kDa according to NP_149019.1. The 22kDa band was successfully blocked by incubation with the immunizing peptide. However, literature reports a 36kDa band in Human and Mouse Testes (PMID: 11390979 ). Have any further splice variants/modified forms been reported?
Application Type
Pep-ELISA, IHC

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