Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names BTB/POZ domain-containing protein 7| MGC48310| FUP1| DKFZp686N0544| BTB (POZ) domain containing 7|BTBD7 | |
Accession ID NP_001002860.2; NP_060637.1 | |
Blocking Peptide EBP10630 | |
Immunogen Peptide with sequence ESKLYSLDHGHEKPQ-C, from the N Terminus of the protein sequence according to NP_001002860.2; NP_060637.1. | |
Product Comments This antibody is expected to recognize both reported isoforms (NP_001002860.2; NP_060637.1). | |
Peptide Sequence ESKLYSLDHGHEKPQ-C | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Pig, Cow | |
Reactive Species Mouse | |
Human Gene ID 55727 | |
Mouse Gene ID 238386 | |
Rat Gene ID 362772 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:64000. | |
Western Blot In transfected MDCK transiently expressing the fusion protein Myc-Btbd7-GFP a band of approx. 190kDa is observed. This band is also observed by Myc-antibody and it is not observed in the MDCK expressing GFP, or when the fusion gene was suppressed. Data provided by Everest Grant winner Melinda Larsen, State University of New York, Albany, NY. This antibody has been successfully used in WB on Mouse: PMID: 31303496. Grisard E et al. EBioMedicine. 2019 Aug;46:79-93. | |
Application Type Pep-ELISA, WB |
Goat Anti-BTBD7 Antibody
$423.00
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Selected References [{"pmid": 31303496, "intro": "This antibody has been successfully used in Western blot on Mouse:", "title": "Sleeping beauty genetic screen identifies miR-23b::BTBD7 gene interaction as crucial for colorectal cancer metastasis.", "author": "Grisard E, Coan M, Cesaratto L, Rigo I, Zandonà L, Paulitti A, Andreuzzi E, Rampioni Vinciguerra GL, Poletto E, Del Ben F, Brisotto G, Biscontin E, Turetta M, Dassi E, Mirnezami A, Canzonieri V, Vecchione A, Baldassarre G, Mongiat M, Spizzo R, Nicoloso MS", "journal": "EBioMedicine. 2019 Aug;46:79-93"}] |