Goat Anti-Caspase 3 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

procaspase3|cysteine protease CPP32|apopain|Yama|SREBP cleavage activity 1|PARP cleavage protease|SCA-1|CPP32B|CPP32|caspase 3, apoptosis-related cysteine peptidase|HGNC:1504|caspase 3, apoptosis-related cysteine protease|caspase 3|CASP3

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 cells. Recommended concentration: 10µg/ml. This antibody has been successfully used in ICC on Rat: Hóngyi Zhào et al. (2018) PMID: 29853981.

NP_004337.2; NP_116786.1

EBP07286

Peptide with sequence C-RDVSKEDHSKRS, from the internal region of the protein sequence according to NP_004337.2; NP_116786.1.

This antibody is expected to recognise both reported isoforms (NP_004337.2 and NP_116786.1).

C-RDVSKEDHSKRS

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog

Human, Mouse, Rat

836

Antibody detection limit dilution 1:32000.

Approx 32kDa band observed in lysates of cell line MOLT4 and in Mouse Liver lysates (calculated MW of 31.6kDa according to Human NP_004337.2 and 31.4kDa according to Mouse NP_033940.1, NP_001271338.1). An additional band of 65kDa was also consistently observed in MOLT4 and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.5-1µg/ml. Primary incubation 1 hour at room temperature.

Pep-ELISA, WB, IF/ ICC