Goat Anti-Catalase / CAT Antibody

$423.00

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SKU: EB07282
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
MGC138424|MGC138422|HGNC:1516|catalase|CAT
Accession ID
NP_001743.1
Blocking Peptide
EBP07282
Immunogen
Peptide with sequence C-DKYNAEKPKNAIHT, from the internal region of the protein sequence according to NP_001743.1.
Peptide Sequence
C-DKYNAEKPKNAIHT
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Dog
Reactive Species
Human, Mouse
Human Gene ID
847
Mouse Gene ID
12359
Product Grade
IHC Results
Paraffin embedded Human Kidney. Recommended concentration: 4-6µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 60kDa band observed in Human and Mouse Kidney and Liver lysates (calculated MW of 59.8kDa according to Human NP_001743.1 and Mouse NP_033934.2 ). Recommended concentration: 0.003-0.01µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC
Selected References
[{"pmid": 27890722, "intro": "This antibody (previous batch) has been successfully used in Western blot on Mouse:", "title": "Diabetes-induced hepatic oxidative stress: a new pathogenic role for glycated albumin.", "author": "Patche J, Girard D, Catan A, Boyer F, Dobi A, Planesse C, Diotel N, Guerin-Dubourg A, Baret P, Bravo SB, Paradela-Dobarro B, Álvarez E, Essop MF, Meilhac O, Bourdon E, Rondeau P.", "journal": "Free Radic Biol Med. 2017 Jan;102:133-148."}, {"pmid": 28543688, "intro": "This antibody (previous batch) has been successfully used in Western blot on Mouse:", "title": "Glycated human albumin alters mitochondrial respiration in preadipocyte 3T3-L1 cells.", "author": "Baret P, Le Sage F, Planesse C, Meilhac O, Devin A, Bourdon E, Rondeau P", "journal": "Biofactors. 2017 Jul 8;43(4):577-592."}]