Goat Anti-Caveolin 1 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

LCCNS|CGL3|BSCL3|cell growth-inhibiting protein 32|caveolin 1, beta isoform|caveolin 1, alpha isoform|caveolin 1 caveolae protein, 22kD|caveolin 1|caveolae protein, 22-kD|VIP21|MSTP085|CAV|HGNC:1527|caveolin 1, caveolae protein, 22kDa|CAV1

Immunofluorescence: Strong expression of the protein seen in the Golgi and membranes of A431 cells and in the cytoplasm and Golgi of U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

NP_001744.2; NP_001166366.1

EBP06817

Peptide with sequence C-DELSEKQVYDAH, from the internal region of the protein sequence according to NP_001744.2; NP_001166366.1.

This antibody is expected to recognize both reported isoforms (NP_001744.2; NP_001166366.1). Reported variants represent identical protein: NP_001166367.1, NP_001166368.1, NP_001166366.1. No cross-reactivity expected to Caveolin 2 and Caveolin 3

C-DELSEKQVYDAH

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog, Pig

Human

857

12389

25404

Paraffin embedded Human Uterus. Recommended concentration: 2.5µg/ml.

Antibody detection limit dilution 1:8000.

Approx 22kDa band observed in lysates of cell lines A431 and A549 and in Human Heart lysates (calculated MW of 20.5kDa according to NP_001744.2). Recommended concentration: 0.001-0.01µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Pig Heart and Spleen, and Mouse and Rat Lung for this particular batch.

Pep-ELISA, WB, IF, IHC, FC