Goat Anti-CD34-T Antibody

$423.00

Category: Primary Antibodies
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SKU: EB11681
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
OTTHUMP00000034734|OTTHUMP00000034733|hematopoietic progenitor cell antigen CD34|CD34-T|CD34 molecule|CD34 antigen|CD34
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the plasma membranes and nuclei of Jurkat and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: A customer reported weak staining of Jurkat cells at a concentration of 10ug/ml.

Accession ID
NP_001764.1
Blocking Peptide
EBP11681
Immunogen
Peptide with sequence C-SPTGERLELEP, from the C-Terminus of the protein sequence according to NP_001764.1.
Product Comments
This antibody is expected to recognize reported isoform CD34-T (NP_001020280.1) only.
Peptide Sequence
C-SPTGERLELEP
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse
Reactive Species
Human
Human Gene ID
947
Mouse Gene ID
12490
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Preliminary testing showed bands at approx 85+90kDa in Human Peripheral Blood Mononucleocytes lysate (calculated MW of 35.0kDa according to NP_001764.1). The observed MW correspond to glycosylated forms, routinely observed by other sources. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, IF

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