Goat Anti-CD4 Antibody

$464.00

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SKU: EB12597
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
T-cell surface glycoprotein CD4|T-cell surface antigen T4/Leu-3|CD4 receptor|CD4 antigen (p55)|CD4mut|CD4 molecule|CD4
Usage Summary
Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
Accession ID
NP_000607.1; NP_001181943.1
Blocking Peptide
EBP12597
Immunogen
Peptide with sequence C-KNKEVSVKRVTQDPK, from the internal region of the protein sequence according to NP_000607.1; NP_001181943.1.
Product Comments
This antibody is expected to recognize isoform 1 (NP_000607.1) and isoform 2 (NP_001181943.1).
Peptide Sequence
C-KNKEVSVKRVTQDPK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
920
Product Grade
IHC Results
Paraffin embedded Human Tonsil. Recommended concentration: 4µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 30kDa band observed in Human Spleen and Bone Marrow lysates (calculated MW of 31.3kDa according to NP_001181943.1). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC, FC