Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names integrin associ|antigenic surface determinant protein OA3|antigen identified by monoclonal antibody 1D8|Rh-related antigen|CD47 glycoprotein|CD47 antigen (Rh-related antigen, integrin-associated signal transducer)|OA3|MER6|IAP|CD47 molecule|CD47 | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the membranes and cytoplasm of A431 and THP-1 cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of THP-1 cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_001768.1; NP_942088.1; NP_001369235.1 | |
Blocking Peptide EBP12502 | |
Immunogen Peptide with sequence C-PTDFSSAKIEVS, from the internal region of the protein sequence according to NP_001768.1; NP_942088.1; NP_001369235.1. | |
Product Comments This antibody is expected to recognize all reported isoforms NP_001768.1; NP_942088.1; NP_001369235.1 | |
Peptide Sequence C-PTDFSSAKIEVS | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human | |
Reactive Species Human | |
Human Gene ID 961 | |
Product Grade ![]() | |
ELISA Detection Limit Antibody detection limit dilution 1:128000. | |
Western Blot Approx. 48kDa band observed in Human Tonsil lysates (calculated MW of 35.2kDa according to NP_001768.1). This molecular weight is observed by other commercial sources, and was blocked by incubation with the immunizing peptide. Recommended concentration: 0.5-2ug/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB, IF, FC |
Goat Anti-CD47 (aa78-89) Antibody
$423.00
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