Goat Anti-CD47 (aa78-89) Antibody

$423.00

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SKU: EB12502
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
integrin associ|antigenic surface determinant protein OA3|antigen identified by monoclonal antibody 1D8|Rh-related antigen|CD47 glycoprotein|CD47 antigen (Rh-related antigen, integrin-associated signal transducer)|OA3|MER6|IAP|CD47 molecule|CD47
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the membranes and cytoplasm of A431 and THP-1 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of THP-1 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_001768.1; NP_942088.1; NP_001369235.1
Blocking Peptide
EBP12502
Immunogen
Peptide with sequence C-PTDFSSAKIEVS, from the internal region of the protein sequence according to NP_001768.1; NP_942088.1; NP_001369235.1.
Product Comments
This antibody is expected to recognize all reported isoforms NP_001768.1; NP_942088.1; NP_001369235.1
Peptide Sequence
C-PTDFSSAKIEVS
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
961
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx. 48kDa band observed in Human Tonsil lysates (calculated MW of 35.2kDa according to NP_001768.1). This molecular weight is observed by other commercial sources, and was blocked by incubation with the immunizing peptide. Recommended concentration: 0.5-2ug/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC