Goat Anti-CD56 / NCAM1 (isoform 2) Antibody

$464.00

Category: Primary Antibodies
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SKU: EB10948
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
neural cell adhesion molecule, NCAM| antigen recognized by monoclonal antibody 5.1H11| NCAM-1| N-CAM-1| NCAM| MSK39| CD56| neural cell adhesion molecule 1|NCAM1
Usage Summary

Flow Cytometry: Flow cytometric analysis of U251 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_851996.2
Blocking Peptide
EBP10948
Immunogen
Peptide with sequence C-KASWTRPEKQETLD, from the internal region of the protein sequence according to NP_851996.2.
Product Comments
This antibody is expected to recognize reported isoform 2 (NP_851996.2) only.
Peptide Sequence
C-KASWTRPEKQETLD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Rat, Pig, Cow
Reactive Species
Human
Human Gene ID
4684
Rat Gene ID
24586
Product Grade
IHC Results
Paraffin embedded Human Adrenal Gland. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 150-170kDa band observed in Human Cerebellum and Frontal Cortex lysates (calculated MW of 94.6kDa according to NP_851996.2). The observed molecular weight corresponds to the glycosylated form and is routinely observed by other sources. Recommended concentration: 0.03-0.3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC, FC

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