Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names costimulatory molecule variant IgV-CD80|costimulatory factor CD80|CD80 antigen (CD28 antigen ligand 1, B7-1 antigen)|CD80 antigen|B-lymphocyte activation antigen B7|LAB7|CD28LG1|CD28LG|CD80 molecule|CD80 | |
Usage Summary Flow Cytometry: Flow cytometric analysis of U937 cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_005182.1 | |
Blocking Peptide EBP09701 | |
Immunogen Peptide with sequence C-HLRVNQTFNWNTTK, from the internal region of the protein sequence according to NP_005182.1. | |
Peptide Sequence C-HLRVNQTFNWNTTK | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human | |
Reactive Species Human | |
Human Gene ID 941 | |
Product Grade  | |
IHC Results Paraffin embedded Human Spleen and Tonsil. Recommended concentration: 4-5µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:4000. | |
Western Blot Approx. 65kDa band observed in lysates of cell lines K562 and U937, and approx. 60kDa in Human Lymph Node, Bone Marrow and Spleen lysates (calculated MW of 33.0kDa according to NP_005182.1). These molecular weights are routinely observed by other sources. Recommended concentration: 0.5-3µg/ml. Primary incubation 1 hour at room temperature. Positive Control: A batch specific positive control lysate is available for this product. Please contact [email protected] for availability. | |
Application Type Pep-ELISA, WB, IHC, FC |
Goat Anti-CD80 Antibody
$464.00
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