Goat Anti-Centromere protein E (aa2593-2604) Antibody

$255.00

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SKU: EB12681
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
protein phosphatase 1, regulatory subunit 61|kinesin-related protein CENPE|kinesin family member 10|centromere-associated protein E|Centromere autoantigen E (312kD)|PPP1R61|KIF10|CENP-E|centromere protein E, 312kDa|CENPE
Accession ID
NP_001804.2; NP_001273663.1
Blocking Peptide
EBP12681
Immunogen
Peptide with sequence KREAHKQVTCEN, from the internal region of the protein sequence according to NP_001804.2; NP_001273663.1.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_001804.2; NP_001273663.1).
Peptide Sequence
KREAHKQVTCEN
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Human Gene ID
1062
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 316kDa according to NP_001804.2. Therefore we cannot recommend an optimal concentration and the antibody is an aspiring product. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Application Type
Pep-ELISA