Goat Anti-CFLAR / FLIP Antibody

$423.00

Category: Primary Antibodies
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SKU: EB05010
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
FADD-like anti-apoptotic molecule Inhibitor of FLICE Caspase-related inducer of apoptosis Caspase homolog Caspase-like apoptosis regulatory protein|USURPIN|I-FLICE|FLAME-1|Casper|CASPER|FLAME|CLARP|MRIT|FLIP|CASH|CASP8 and FADD-like apoptosis regulator|CFLAR
Accession ID
NP_003870.4; NP_001189445.1; NP_001189446.1; NP_001189444.1
Immunogen
Peptide with sequence C-QHTLRKKLILSYT, from the C Terminus of the protein sequence according to NP_003870.4; NP_001189445.1; NP_001189446.1; NP_001189444.1.
Product Comments
This antibody is expected to recognise isoform 1 (NP_003870.4), isoform 3 (NP_001189444.1), isoform 4, (NP_001189445.1) and isoform 5 (NP_001189446.1). Reported variants represent identical protein: NP_003870.4, NP_001120655.1.
Peptide Sequence
C-QHTLRKKLILSYT
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
8837
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:4000.
Western Blot
Western blot: Multiple bands between 55kDa and 35kDa seen in Hela and Jurkat lysate. Recommended for use at 0.1-1 µg/ml. Please note that we consistently see multiple bands between 35 and 55kDa in HeLa and Jurkat whole cell lysates. Given the peptide sequnece used to make this antibody, we expect it to detect major splice varients of FLIP including FLIP alpha, beta, gamma, and delta [Wallach D. Nature (1997) 388:123].
Application Type
Pep-ELISA, WB

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