Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names CHCHD3|FLJ20420|coiled-coil-helix-coiled-coil-helix domain containing 3 | |
Usage Summary Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. | |
Accession ID NP_060282.1 | |
Blocking Peptide EBP09840 | |
Immunogen Peptide with sequence C-RSSEFYRVTTEQYQ, from the internal region of the protein sequence according to NP_060282.1. | |
Peptide Sequence C-RSSEFYRVTTEQYQ | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Cow, Sheep | |
Reactive Species Human | |
Human Gene ID 54927 | |
Mouse Gene ID 66075 | |
Rat Gene ID 296966 | |
Product Grade  | |
IHC Results Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:32000. | |
Western Blot Approx 26.2kDa band observed in Human Placenta lysates (calculated MW of 26kDa according to NP_060282.1). Recommended concentration: 0.3-1µg/ml. A customer reported ~30kDa in THP1 and in human primary monocyte derived dendritic cells. | |
Application Type Pep-ELISA, WB, IHC |
Goat Anti-CHCHD3 (aa151-164) Antibody
$423.00
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Selected References [{"pmid": 30377371, "intro": "This antibody has been successfully used in the following paper:", "title": "A high-throughput pipeline for validation of antibodies", "author": "Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen", "journal": "Nat Methods. 2018 Nov;15(11):909-912"}] |