Goat Anti-CHFR Antibody

$423.00

Category: Primary Antibodies
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SKU: EB05819
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
RING finger protein 196|E3 ubiquitin-protein ligase CHFR|RNF196|RNF116|FLJ33629|FLJ10796|checkpoint with forkhead and ring finger domains|CHFR
Accession ID
NP_001154816.1; NP_001154817.1; NP_001154818.1; NP_060693.2; NP_001154819.1
Blocking Peptide
EBP05819
Immunogen
Peptide with sequence KFNHICEQTRFKN, from the C Terminus of the protein sequence according to NP_001154816.1; NP_001154817.1; NP_001154818.1; NP_060693.2; NP_001154819.1.
Product Comments
This antibody is expected to recognise all reported isoforms.
Peptide Sequence
KFNHICEQTRFKN
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Pig Cow
Reactive Species
Human
Human Gene ID
55743
Mouse Gene ID
231600
Rat Gene ID
288734
Product Grade
IHC Results
In paraffin embedded Human Brain (Cerebellum) shows stained Purkinje cells. Recommended concentration: 2.5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Preliminary experiments gave an approx 80kDa band in human brain lysates at 0.5ug/ml, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 72kDda according to NP_060693.
Application Type
Pep-ELISA, IHC

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