Goat Anti-CHRNA4 (aa29-43) Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

neuronal nicotinic acetylcholine receptor alpha-4 subunit|neuronal acetylcholine receptor alpha 4 subunit|cholinergic receptor, nicotinic, alpha polypeptide 4|cholinergic receptor, nicotinic, alpha 4 subunit|NACRA4|EBN1|EBN|BFNC|cholinergic receptor, nicotinic, alpha 4|CHRNA4

This antibody was shown unfit for immune precipitation.

NP_000735.1

EBP08852

Peptide with sequence C-HVETRAHAEERLLKK, from the internal region of the protein sequence according to NP_000735.1.

This antibody is expected NOT to cross-react with the similar alpha 2 subunit.

C-HVETRAHAEERLLKK

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog, Cow

Human, Rat

1137

11438

25590

Antibody detection limit dilution 1:64000.

Approx 70kDa band observed in Rat Brain lysates (calculated MW of 70.0kDa according to Human NP_000735.1and of 70.2kDa according to Rat NP_077330.1). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? ~75kDa confirmed in both human and rat tissues as the glycoprotein by an anonymous customer.

Pep-ELISA, WB