Goat Anti-CIAS1 / Cryopyrin (Internal) Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

cryopyrin|angiotensin/vasopressin receptor AII/AVP-like|PYRIN-containing APAF1-like protein 1|NACHT, LRR and PYD containing protein 3|Muckle-Wells syndrome|AII/AVP receptor-like|PYPAF1|NALP3|MWS|FCU|FCAS|C1orf7|AII/AVP|AGTAVPRL|cold autoinflammatory syndrome 1|CIAS1

NP_004886.3; NP_899632.1; NP_001230062.1; NP_001120933.1; NP_001120934.1

EBP07335

Peptide with sequence C-DLYEKAKRDEPK, from the internal region of the protein sequence according to NP_004886.3; NP_899632.1; NP_001230062.1; NP_001120933.1; NP_001120934.1.

This antibody is expected to recognise all five reported isoforms (NP_004886.3; NP_899632.1; NP_001230062.1; NP_001120933.1; NP_001120934.1). Reported variants represent identical protein (NP_004886.3 and NP_001073289.1).

C-DLYEKAKRDEPK

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog, Pig, Cow

Human

114548

In paraffin embedded Human Tonsil shows focal and interstitial staining of the inflammatory elements close to the epithelia of the tonsil crypts. Recommended concentration: 2-4µg/ml.

Antibody detection limit dilution 1:64000.

Preliminary experiments gave an approx 70kDa band in human lymph node and tonsil lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 118kDa according to NP_004886.3 and 106kDa according to NP_899632.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide.

Pep-ELISA, IHC