Goat Anti-CLPB Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

CLPB|ClpB caseinolytic peptidase B homolog (E. coli)|FLJ13152|HSP78|SKD3|suppressor of potassium transport defect 3

NP_110440.1

EBP07768

Peptide with sequence C-DKDSKTRRLDIRAP, from the C Terminus of the protein sequence according to NP_110440.1.

C-DKDSKTRRLDIRAP

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human

81570

Antibody detection limit dilution 1:32000.

Preliminary experiments gave bands at approx 50kDa and 48kDa in lysates of HeLa and HepG2 after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 78.7kDa according to NP_110440.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA