Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names catechol-O-methyltransferase|COMT | |
Usage Summary Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_000745.1; NP_001128633.1; NP_001128634.1; NP_009294.1 | |
Blocking Peptide EBP06595 | |
Immunogen Peptide with sequence GDTKEQRILNHVLQC, from the N Terminus of the protein sequence according to NP_000745.1; NP_001128633.1; NP_001128634.1; NP_009294.1. | |
Product Comments This antibody is expected to recognise both reported isoforms S-COMT NP_009294.1 and MB-COMT NP_000745.1; NP_001128633.1; NP_001128634.. | |
Peptide Sequence GDTKEQRILNHVLQC | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Dog | |
Reactive Species Human | |
Human Gene ID 1312 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:4000. | |
Western Blot Approx 25kDa band observed in Human Liver and Ovary lysates and in MCF7 cell lysates, and approx. 25kDa and 30kDa in U251 cell lysate (calculated MW of 30.0kDa according to NP_000745.1(MB COMT) and 24.5kDa according to NP_009294.1 (S-COMT). Recommended concentration: 0.3-2µg/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB, FC |
Goat Anti-COMT (N Terminus) Antibody
$423.00
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Selected References [{"pmid": 30377371, "intro": "This antibody has been successfully used in the following paper:", "title": "A high-throughput pipeline for validation of antibodies", "author": "Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen", "journal": "Nat Methods. 2018 Nov;15(11):909-912"}] |